PCR amplification was performed in a total volume of 10 μl, containing 1 μl cDNA, 1 μl 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U ExTaq polymerase (Takara Bio, Inc.), 500 nM each ...

High tumor mutation burden (TMB) in many cancer types is associated with the production of tumor-specific neoantigens, a favorable outcome and response to immune checkpoint blockade (ICB) therapy.

The interrupted non-coding regions in pre-mRNAs, termed “introns,” are excised by “splicing” to generate mature coding mRNAs that are translated into proteins. As human pre-mRNA introns vary in length ...

Myelodysplastic syndromes (MDS) encompass a heterogeneous group of haematologic disorders collectively defined by aberrant differentiation of myeloid precursors in the bone marrow 1,2. Because of the ...