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To determine the semi-conservative nature of DNA replication, Meselson and Stahl performed the following experiment: Cells were grown in 15N for several generations and then shifted to 14N medium: Samples of cells were taken before the shift to 14N (generation 0) and then after multiple rounds of replication in 14N. The DNA was isolated from cells and subjected to centrifugation in gradient: The following results were obtained after 2 rounds of replication Top of centrifuge tube (lower density)
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